Antimicrobial resistance (AMR) is classified by the World Health Organisation (WHO) as one of the top global public health and development threats and contributes to deaths either directly or indirectly. A 2022 study reviewed data from 2019 and estimated that AMR was directly responsible for 1.27 million deaths and contributed to 4.95 million deaths globally (1). A one health approach coordinating actions across sectors including human and animal health, food production, and environmental are essential to mitigate AMR spread and effects. Essential to control is accurate surveillance and development of novel therapies for treatment of microbial infections. Next-Generation Sequencing (NGS) enables population studies for AMR gene profiling as well as investigations into novel therapies to combat infections. This presentation discusses the use of NGS to combat AMR with case studies for population AMR tracking, novel therapies, and one health approaches.