Poster Presentation Australian Society for Microbiology Annual Scientific Meeting 2024

A novel tamanavirus (Flaviviridae) of the European common frog (Rana temporaria) from the UK (#73)

Rhys H. Parry 1 , Andrii Slonchak 1 , Lewis J. Campbell 2 , Natalee D. Newton 1 , Humberto J. Debat 3 , Robert J. Gifford 4 , Alexander A. Khromykh 1
  1. School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia, QLD, Australia
  2. Institute of Zoology, Zoological Society of London, London, UK
  3. Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), Córdoba X5020ICA, Argentina
  4. Centre for Virus Research, University of Glasgow, Glasgow, UK

Flavivirids are small, enveloped, positive-sense RNA viruses from the family Flaviviridae with genomes of ~9–13kb. Metatranscriptomic analyses of metazoan organisms have revealed a diversity of flavivirus-like or flavivirid viral sequences in fish and marine invertebrate groups. However, no flavivirus-like virus has been identified in amphibians. To remedy this, we investigated the virome of the European common frog (Rana temporaria) in the UK, utilizing high-throughput sequencing at six catch locations. De novo assembly revealed a coding-complete virus contig of a novel flavivirid ~11.2kb in length. The virus encodes a single ORF of 3456 aa and 5′ and 3′ untranslated regions (UTRs) of 227 and 666 nt, respectively. We named this virus Rana tamanavirus (RaTV), as BLASTp analysis of the polyprotein showed the closest relationships to Tamana bat virus (TABV) and Cyclopterus lumpus virus from Pteronotus parnellii and Cyclopterus lumpus, respectively. Phylogenetic analysis of the RaTV polyprotein compared to Flavivirus and Flavivirus-like members indicated that RaTV was sufficiently divergent and basal to the vertebrate Tamanavirus clade. In addition to the Mitcham strain, partial but divergent RaTV, sharing 95.64–97.39%pairwise nucleotide identity, were also obtained from the Poole and Deal samples, indicating that RaTV is widespread in UK frog samples. Bioinformatic analyses of predicted secondary structures in the 3′UTR of RaTV showed the presence of an exoribonuclease-resistant RNA (xrRNA) structure standard in flaviviruses and TABV. To examine this biochemically, we conducted an in vitro Xrn1 digestion assay showing that RaTV probably forms a functional Xrn1-resistant xrRNA.